Culture

Collagen is a protein found widely in almost all cells of animals, and scientifically can be used to learn much about an animal's life history including human being in the present or in the past. Scientists at the Research Institute for Humanity and Nature (RIHN) and Japan Fisheries Research and Education Agency (FRA), Japan, prove this point for Japanese flounder by measuring isotope ratios in vertebral-bone collagen. The new study, which can be read in Marine Biology, shows that there exist behavioral groups of fish with different migrating and/or feeding patterns.

A school of fish will decide their habitat on fundamental needs for survival, such as food availability and safety for reproduction and nursing offspring. Multiple methods have been applied for tracing fish movement, such as telemetry, tagging or analysis of otolith. Examining the stable isotope ratios in several body parts, however, provides unique insights on fish life history.

"Juveniles are interesting, because this is the stage when the fish leave the nursery and begin exploring deeper parts of the sea. Even in adults, however, life-span records are well preserved in their vertebral centrum, cone-shaped layered structure in fish vertebra, as the form of stable isotope ratios of several elements. It allows us to reconstruct individual migration and feeding history," said RIHN ex-researcher Dr. Yoshikazu Kato, the lead author of the study.

Kato and his colleagues examined a wild population of Japanese flounder (aralichthys olivaceus) in Sendai Bay, off the Pacific coast of northern Japan. The fish was chosen for their important commercial status in Japan.

The migratory and feeding habits of the fish have been well studied in the past, but little is known about how their habits change with age and are diverse among individuals that belongs genetically uniformed group.

Adult fishes were collected at four sampling sites in the bay. Then, carbon and nitrogen stable isotopes of collagen in their vertebral centrum were analyzed chronologically. A non-linear time series analysis using both stable isotope ratios distinguished fishes collected at a site from fishes at the other three sites, suggesting these fishes used a different habitat and/or diet throughout most of their lifetimes. By analyzing stable isotope ratios of nitrogen in amino acids, Kato and his colleagues also detected migrations from shallow nursery to deeper habitats and changes of their foods.

These findings, Kato notes, suggest that when considering effective management and conservation policies, scientists should recognize that not all Japanese flounder will respond the same.

"Our findings indicated that Paralichthys olivaceus are not just a homogenous group in behavior but of at least two different groups who have different migration patterns. As their habitat changes, either naturally or through man-made effects, we expect these behavioral groups will respond differently," he said.

Studying the violent collisions of black holes and neutron stars may soon provide a new measurement of the Universe's expansion rate, helping to resolve a long-standing dispute, suggests a new simulation study led by researchers at UCL (University College London).

Our two current best ways of estimating the Universe's rate of expansion - measuring the brightness and speed of pulsating and exploding stars, and looking at fluctuations in radiation from the early Universe - give very different answers, suggesting our theory of the Universe may be wrong.

A third type of measurement, looking at the explosions of light and ripples in the fabric of space caused by black hole-neutron star collisions, should help to resolve this disagreement and clarify whether our theory of the Universe needs rewriting.

The new study, published in Physical Review Letters, simulated 25,000 scenarios of black holes and neutron stars colliding, aiming to see how many would likely be detected by instruments on Earth in the mid- to late-2020s.

The researchers found that, by 2030, instruments on Earth could sense ripples in space-time caused by up to 3,000 such collisions, and that for around 100 of these events, telescopes would also see accompanying explosions of light.

They concluded that this would be enough data to provide a new, completely independent measurement of the Universe's rate of expansion, precise and reliable enough to confirm or deny the need for new physics.

Lead author Dr Stephen Feeney (UCL Physics & Astronomy) said: "A neutron star is a dead star, created when a very large star explodes and then collapses, and it is incredibly dense - typically 10 miles across but with a mass up to twice that of our Sun. Its collision with a black hole is a cataclysmic event, causing ripples of space-time, known as gravitational waves, that we can now detect on Earth with observatories like LIGO and Virgo.

"We have not yet detected light from these collisions. But advances in the sensitivity of equipment detecting gravitational waves, together with new detectors in India and Japan, will lead to a huge leap forward in terms of how many of these types of events we can detect. It is incredibly exciting and should open up a new era for astrophysics."

To calculate the Universe's rate of expansion, known as the Hubble constant, astrophysicists need to know the distance of astronomical objects from Earth as well as the speed at which they are moving away. Analysing gravitational waves tells us how far away a collision is, leaving only the speed to be determined.

To tell how fast the galaxy hosting a collision is moving away, we look at the "redshift" of light - that is, how the wavelength of light produced by a source has been stretched by its motion. Explosions of light that may accompany these collisions would help us pinpoint the galaxy where the collision happened, allowing researchers to combine measurements of distance and measurements of redshift in that galaxy.

Dr Feeney said: "Computer models of these cataclysmic events are incomplete and this study should provide extra motivation to improve them. If our assumptions are correct, many of these collisions will not produce explosions that we can detect - the black hole will swallow the star without leaving a trace. But in some cases a smaller black hole may first rip apart a neutron star before swallowing it, potentially leaving matter outside the hole that emits electromagnetic radiation."

Co-author Professor Hiranya Peiris (UCL Physics & Astronomy and Stockholm University) said: "The disagreement over the Hubble constant is one of the biggest mysteries in cosmology. In addition to helping us unravel this puzzle, the spacetime ripples from these cataclysmic events open a new window on the universe. We can anticipate many exciting discoveries in the coming decade."

Gravitational waves are detected at two observatories in the United States (the LIGO Labs), one in Italy (Virgo), and one in Japan (KAGRA). A fifth observatory, LIGO-India, is now under construction.

Our two best current estimates of the Universe's expansion are 67 kilometres per second per megaparsec (3.26 million light years) and 74 kilometres per second per megaparsec.
The first is derived from analysing the cosmic microwave background, the radiation left over from the Big Bang, while the second comes from comparing stars at different distances from Earth - specifically Cepheids, which have variable brightness, and exploding stars called type Ia supernovae.

Dr Feeney explained: "As the microwave background measurement needs a complete theory of the Universe to be made but the stellar method does not, the disagreement offers tantalising evidence of new physics beyond our current understanding. Before we can make such claims, however, we need confirmation of the disagreement from completely independent observations - we believe these can be provided through black hole-neutron star collisions."

The study was carried out by researchers at UCL, Imperial College London, Stockholm University and the University of Amsterdam. It was supported by the Royal Society, the Swedish Research Council (VR), the Knut and Alice Wallenberg Foundation, and the Netherlands Organisation for Scientific Research (NWO).

Oxytocin and arginine vasopressin are two hormones in the endocrine system that can act as neurotransmitters and regulate -in vertebrates and invertebrates- a wide range of biological functions, such as bonding formation, breastfeeding, birth or arterial pressure. Biochemists in the pregenomic era, named these genes differently in different species, due to small protein coding differences.

A new study carried out by the University of Barcelona (UB) and the Rockefeller University, published in the journal Nature, has analysed and compared the genome of 35 species representing all vertebrate lineages and reached the conclusion that both hormones stem from a common ancestral gene.

These high-quality genomes were obtained thanks to the use of innovative genome sequencing technologies in the context of the Vertebrate Genomes Project. Based on these findings, the researchers propose a new universal nomenclature, based on their identified evolutionary history for these genes. According to the authors of the study, the proposal allows for a new nomenclature that is universal across vertebrates, both for the hormone-genes and their receptors, therefore easing the species comparative research.

The paper is part of the doctoral thesis by Constantina Theofanopoulou, carried out at the UB under the co-supervision of Cedric Boeckx, ICREA researcher at the Institute of Complex Systems of the UB (UBICS) and Erich D. Jarvis, professor at the Rockefeller University.

Terminological confusion between species

The study results from problems the first author of the study, Constantina Theofanopoulou, became aware of in the literature of these genes, when analysing the role of oxytocin in the vocal learning ability in songbirds. "When analysing the scientific literature to find previous work on this gene, we observed that when talking about birds, scientists used the term 'mesotocin', instead of 'oxytocin'. Different names, like 'isotocin' or 'neurophysin' were used in other vertebrate species, such as turtles, frogs or fish. An equally confusing nomenclature was the one used for their respective receptors, making it very difficult to understand which gene is which in different species and lineages", says Constantina Theofanopoulou. In this context, the researchers aimed to find out which genes were truly orthologous to each other in different species. To do so, they used the genome assemblies generated by the Vertebrates Genome Project, an international collaboration led by Erich Jarvis, from the Rockefeller University. In the first phase, Theofanopoulou and the research team compared the genomes and specifically the order of genes around their genes of interest. They studied up to sixty genes around the genes of interest, as well as all the genes of the chromosomes where these genes are located. They sought to find out whether the order of genes -called synteny- was conserved across species, something that would shed light to the evolutionary history of these genes.

The new high-quality assemblies were more than helpful since, as Theofanopoulou says, "Most genomes so far have had low quality, meaning millions of mistakes, parts of genes being absent or incorrectly assembled. These errors can have direct consequences on the scientific findings".

Genes with a common ancestor

The results, together with other phylogenetic analyses carried out during the study, show that genes that the researchers of this study now call oxytocin and vasotocin -that code for the homonymous hormones- are paralogous, that is, they come from a local duplication of a common ancestral gene. "Our findings suggest that the oxytocin gene is a duplication of the vasotocin gene -historically called vasopressin- that took place after the divergence of jawed vertebrates from jawless vertebrates. This simultaneously means that, if we had understood the evolution of these genes from the very beginning, we would most likely not have used different names to refer to them, as we see in other gene families, where we use the same root name for all its gene-members (e.g., FOXP) and different numbers to differentiate them (e.g., FOXP1, FOXP2 etc.)", highlights Constantina Theofanopoulou.

Given the results, the researchers propose a universal nomenclature in which oxytocin and vasotocin are used for these genes in all jawed vertebrates, and vasotocin for the only gene present all jawless vertebrates and closely related invertebrates. In this proposal, the common origin of both genes would be represented through the shared suffix -tocin, while the parology would be displayed through the different prefixes (oxy- and ¬vaso-). Following this new proposed nomenclature, what is currently called oxytocin, mesotocin, isotocin, glumitocin, valitocin, aspargtocin and neurophysin in different lineages, would be now called oxytocin universally. As for what is currently named vasotocin, vasopressin, neurophysin 2 and phenypresin in different species, would now be called vasotocin for all species.

This proposal also includes a universal nomenclature for the receptors of both genes, being oxytocin and vasotocin receptors (OTR and VTRs). Therefore, what has traditionally been called oxytocin receptor (OXTR) in mammals, vasotocin 3 receptor (VT3) and mesotocin receptor (MTR) in birds and frogs, and isotocin receptor (ITR) in fish, would be now called oxytocin receptor (OTR) in all cases.

Evolutionary history of oxytocin and vasotocin

The study also identified six main receptors of oxytocin and vasotocin in jawed vertebrates, with two of them being present already in the hagfish, two additional in lampreys, which have four receptors in total, and two more in jawed, which display six receptors in total. This pattern suggests that these receptors have first evolved through one round of whole genome duplication that gave rise to the four receptors we find in jawless vertebrates, like the lampreys, and that afterwards either smaller-scale segmental duplications or another whole genome duplication gave rise to the six receptors in jawed vertebrates.

This is the second time researchers find evidence for a scenario with one round of whole genome duplication, instead of two. The hypothesis that vertebrate genomes have evolved through two rounds of whole genome duplication has not been challenged in almost fifty years. "Our data show more support for the one round of whole genome duplication-idea. If the two rounds of whole genome duplication were the case, we would expect to find eight in total and not six receptors in jawed vertebrates. Of course, gene loss is very common, so that's why we can't exclude a two round-scenario, but we did not find any genomic sign of gene loss in any of the jawed vertebrate genomes we studied", notes the researcher.

The next step would be to get this new nomenclature, which is based on the evolutionary history of genes, adopted. "This is the first time that such a universal change affecting so many genes and species is being presented. Regardless of whether this proposal will be adopted or not, researchers will now have a guide on the genetic orthologies among species, which will make their research much easier", concludes Constantina Theofanopoulou.

According to the researchers, three songs from a playlist are enough to identify the person who chose the songs. Hence, companies like YouTube and Spotify can accumulate a great deal of information about their users based only on their musical preferences.

The study was led by Dr. Ori Leshman of the Jaime and Joan Constantiner School of Education at Tel Aviv University and Dr. Ron Hirschprung of the Department of Management and Industrial Engineering at Ariel University. The study was published in the journal Telematics and Informatics.

The study included about 150 young people (all undergraduate students), in 4 groups of about 35 people each. Participants were asked to identify group members based on only three songs from their favorite playlist. The variety of the students' musical preferences was wide and very diverse, including, for example, both old and new Israeli music (from Sasha Argov to Kaveret, Zohar Argov, Omer Adam and Hanan Ben Ari), classic rock and pop (from the Beatles and Pink Floyd to Beyonce and Ariana Grande), Israeli and international hip hop (from Kendrick Lamar and Eminem to Hadag Nahash and Tuna) and more. The analysis of the choices was carried out according to a mathematical model developed by the scholars.

The findings surprised even the researchers. The analysis of the data showed that the group members were able to identify the study participants according to their musical taste at a very high level of between 80 and 100%, even though the group members did not know each other well and had no prior knowledge of each other's musical preferences.

Dr. Leshman and Dr. Hirschprung explain: "Music can become a form of characterization, and even an identifier. It provides commercial companies like Google and Spotify with additional and more in-depth information about us as users of these platforms. In the digital world we live in today, these findings have far-reaching implications on privacy violations, especially since information about people can be inferred from a completely unexpected source, which is therefore lacking in protection against such violations. Visiting YouTube is perceived by the ordinary person as an innocuous act, but this study shows that it can reveal a lot about that person. On the other hand, this knowledge can be used as a bridge between people and perhaps in the future lead to the creation of new diagnostic methods and fascinating intervention programs that will make use of people's favorite music."

PITTSBURGH, April 28, 2021 - Asthma exacerbations rose following a catastrophic Christmas Eve fire two years ago that destroyed pollution controls at the Clairton Coke Works--the largest such facility in the nation, a University of Pittsburgh Graduate School of Public Health analysis concludes.

The study, published in the Journal of Allergy and Clinical Immunology, was possible because of a collaboration with the University of Pittsburgh Asthma and Environmental Lung Health Institute at UPMC and the Allegheny County Health Department, with funding from The Heinz Endowments.

"In addition to verifying that people living within a 10-mile radius of the coke works had higher rates of asthma exacerbations and use of albuterol rescue medication than those living outside the radius, we learned that nearly half of the people with asthma closest to the fire were unaware of the pollution problem and, therefore, unable to take steps to avoid exposure," said lead author Brandy Byrwa-Hill, M.S., a Ph.D. student in Pitt Public Health's Department of Environmental and Occupational Health.

The Clairton Coke Works is located in a suburb south of Pittsburgh and produces highly refined coal, or "coke," which is used as fuel in the manufacture of steel. Creating coke results in several air pollutants, which are minimized through the plant's pollution controls. On Dec. 24, 2018, a fire destroyed the pollution controls, and, for 102 days, the plant emitted sulfur dioxide at levels 25 times greater than typical emissions.

Byrwa-Hill and the Pitt Public Health team used the Pitt Asthma Institute Research (AIR) registry to quickly collect information from 39 asthma patients living within 10 miles of the coke works and 44 patients living beyond that radius in the six weeks after the fire.

During the pollution control breach, participants who lived closest to the plant had an 80% increased risk of worsened asthma symptoms compared with those furthest from the plant. The difference normalized after the plant was repaired.

Despite news reports and alerts from the Allegheny County Health Department urging people with certain health conditions to take precautions, 44% of the participants were unaware of the excessive pollution.

"When we asked the participants if they would want to know about an environmental disaster, of course they said they would," said senior author James Fabisiak, Ph.D., associate professor of environmental and occupational health and director of the Center of Health Environments and Communities at Pitt Public Health. "Our study reveals that there is a need for a more robust notification system that uses many modes of communication so people can make informed, timely decisions to protect their health."

In addition, the study highlighted the benefit of having a pre-existing registry of well-characterized, geographically identified asthma patients willing to participate in research, said co-senior author Sally Wenzel, M.D., chair of Pitt Public Health's Department of Environmental and Occupational Health.

"I'd encourage any city or county that is home to a significant point source of air pollution to create a similar registry," said Wenzel, who also directs Pitt's Asthma and Environmental Lung Health Institute at UPMC. "People with asthma are particularly sensitive to air pollution, and their experience can be informative to all of us when it comes to maintaining healthy air quality."

Researchers have discovered an explanation for why cerebral cavernous malformations (CCMs)--clusters of dilated blood vessels in the brain--can suddenly grow to cause seizures or stroke. Specifically, they found that a specific, acquired mutation in a cancer-causing gene (PIK3CA) could exacerbate existing CCMs in the brain. Furthermore, repurposing an already existing anticancer drug showed promise in mouse models of CCMs in improving brain-vascular health and preventing bleeding into the brain tissue.

Previous studies linked the initial formation of CCMs to various environmental factors, including differences in the gut microbiome, and inactivating mutations in three specific genes collectively known as the "CCM complex." While these changes are enough to cause small malformations to form in the brain, they didn't explain why some suddenly expand in size, resulting in seizures or stroke.

Using mouse genetic models of CCM formation, the researchers discovered that it is the additional "hit" that stimulates the known cancer-causing gene PIK3CA and leads to the rapid growth of existing CCMs. When they examined resected human CCM tissue, they saw the same genes were involved, which supports the idea of a "cancer-like" mechanism for accelerated blood vessel malformation growth in which small quiescent CCMs become "malignant" after a new gene mutation occurs.

In cancer, the PIK3CA mutation results in an increase in PI3K-mTOR signaling, which is a well-established drug target for the treatment of tumors. Rapamycin is an FDA-approved drug that inhibits that same signaling pathway and has been used to treat malformations in the veins and lymphatic system. Here, rapamycin significantly reduced CCM formation in genetic mouse models, suggesting it could be potentially used as a treatment.

The study was led by Mark L. Kahn, M.D. at the Perelman School of Medicine, University of Pennsylvania, Philadelphia. His team continues to study what causes CCM formation and growth, and proposes that further analyses of human CCM lesions and clinical testing of rapamycin and similar drugs is necessary to determine whether this mechanism can be a target for therapy.

A comprehensive study from Uppsala University demonstrates that socioeconomic deprivation modifies genetic effects on higher education and abstract reasoning. The paper illustrates how genes play a greater role in educational attainment in more socioeconomically deprived regions of the United Kingdom. The study was recently published in the American Journal of Psychiatry.

Education is an important factor in an individual's life and strongly linked to economic outcomes and quality of life. The likelihood of completing higher education is partly determined by genetic factors. Common genetic variants have previously been estimated to contribute 11-13% of the variance in whether an individual completes higher education. This heritability is an estimate of how much a trait is influenced by genes.

In the current study, scientists at Uppsala University assessed the heritability of higher educational attainment and performance on a verbal and numerical reasoning test. Heritability was compared across the socioeconomic spectrum in more than 350,000 participants in the UK Biobank cohort.

The researchers found that common genetic variants contributed 13% of the variance in the least socioeconomically deprived, but almost 26% in the most deprived. "We were surprised to find that the heritability was higher in participants from more socioeconomically deprived regions of the UK. This is the opposite of what previous twin studies have found," says the lead author of the study, Dr Mathias Rask-Andersen. This type of difference in heritability across the socioeconomic spectrum is likely to represent an interaction between genes and the environment, where socioeconomic deprivation modifies genetic effects.

Previous studies in the field have mainly been performed in small twin cohorts from the United States. According to Dr Rask-Andersen, the contrasting results of the current study indicate that national differences may influence genetic effects. For instance, differences in access to and quality of education across the socioeconomic spectrum may play an important role, as well as welfare support systems and healthcare.

The current study illustrates how the environment determines how an individual's genetic makeup is propagated. "More and more studies demonstrate that environmental factors influence genetic effects," explains Dr Åsa Johansson, group leader at the Department of Immunology, Genetics and Pathology at Uppsala University. "We have previously seen similar gene-environment interactions for other traits, such as body mass index, and it is likely that interactions also exist for many human diseases."

In the long run, gene-environment interactions offer an additional layer of information on the contribution of genetics to human traits and diseases. Identifying these interactions can potentially lead to more precise forms of interventions, not only for preventing and treating disease, but also for increasing the likelihood of successful educational outcomes.

The results from this study also have implications for notions of equality of outcome and opportunity. "The larger influence of genetic factors in more socioeconomically deprived regions could reflect greater challenges for higher education in these regions," says Dr Rask-Andersen. "Someone who is more vulnerable due to genetic factors would then face even greater challenges in a more socioeconomically deprived region."

How the study was conducted:

UK Biobank is a cross-sectional cohort of almost half a million UK residents aged 40-70 years old who were recruited in 2006-2010. Participants answered extensive questionnaires and were tested for abstract verbal and numerical reasoning. Educational attainment was determined from the self-reported professional qualifications of each participant. The level of socioeconomic deprivation for each participant was assessed from census data. Each participant was thus assigned a score for socioeconomic deprivation based on their place of residence. Each participant was genotyped and the effects of genetic variants on educational attainment and abstract reasoning were determined by association tests, providing an estimate of the effect of each genetic variant on educational attainment and abstract reasoning. The researchers divided the cohort into five quintiles based on socioeconomic deprivation and performed association tests in each quintile. The heritability in each quintile was then estimated by examining how much the genetic effects contributed to the outcomes. The researchers then compared heritabilities between quintiles.

The degree of acidity or alkalinity of a substance is crucial for its chemical behavior. The decisive factor is the so-called proton affinity, which indicates how easily an entity accepts or releases a single proton. While it is easy to measure this for molecules, it has not been possible for surfaces. This is important because atoms on surfaces have very different proton affinities, depending on where they sit.
Researchers at TU Wien have now succeeded in making this important physical quantity experimentally accessible for the first time: Using a specially modified atomic force microscope, it is possible to study the proton affinity of individual atoms. This should help to analyze catalysts on an atomic scale. The results have been published in the scientific journal Nature.

Precision instead of average

"All previous measurements of surface acidity had one severe drawback," says Prof. Ulrike Diebold from the Institute of Applied Physics at TU Wien. "Although the surface atoms behave chemically differently, one could only ever measure the average value."

Thus it is not known which atoms contributed to chemical reactions, and to what extent, which makes it impossible to adjust the atomic scale of the surface to favor certain chemical reactions. But that is exactly what is needed, for example, when looking for more effective catalysts for hydrogen production.

"We analyzed surfaces made of indium oxide. They are particularly interesting because there are five different types of OH groups with different properties on the surface," says Margareta Wagner, who carried out these measurements in Prof. Diebold's lab.

With a special trick it was possible to study these OH groups individually: The researchers placed a single OH group at the tip of an atomic force microscope. This tip was then positioned specifically over one particular atom on the surface. A force then acts between the OH group on the tip and the OH group directly below it on the indium oxide surface, and this force depends sensitively on the distance between them.

"We vary the distance between the tip and the surface and measure how this changes the force," explains Margareta Wagner. "This gives us a characteristic force curve for each OH group on the surface of a material." The shape of this force curve provides information about how well the respective oxygen atoms on the indium oxide surface hold their protons - or how easily they will release them.

In order to obtain an actual value for the proton affinity, theoretical work was necessary. This was carried out by Bernd Meyer at the Friedrich-Alexander-University Erlangen-Nürnberg, Germany. In elaborate computer simulations it was possible to show how the force curve of the atomic force microscope can be translated in a simple and precise way into those quantities that are needed in chemistry.

Nanostructure determines the quality of catalysts

"This is quite crucial for the further development of catalysts," says Bernd Meyer. "We know that atoms of the same type behave quite differently depending on their atomic neighbors and the way they are incorporated into the surface." For example, it can make a big difference whether the surface is perfectly smooth or has steps on an atomic scale. Atoms with a smaller number of neighbors sit at such step edges, and they can potentially significantly improve or worsen chemical reactions.

"With our functionalized scanning force microscope tip, we can now precisely investigate such questions for the first time," says Ulrike Diebold. "This means that we no longer have to rely on trial and error, but can precisely understand and improve chemical properties of surfaces."

Breast cancer diagnosis: Around 88 percent of patients survive the dangerous disease in the first five years. Work is important for getting back to normality. Researchers from the University of Bonn and the German Cancer Society investigated how satisfied former patients are with their occupational development over a period of five to six years since diagnosis. About half experienced at least one job change during the study period. Around ten percent of those affected even report involuntary changes. The researchers conclude that there is a need for long-term support measures for patients. The study is now published in the Journal of Cancer Survivorship.

Breast cancer is the most commonly occurring cancer in women. Almost 70,000 cases are diagnosed every year in Germany alone. Studies show that the five-year survival rate is 88 percent. "Returning to work is important; it provides a sense of normality and meaning after a crisis caused by cancer," explains sociologist Kati Hiltrop from the Center for Health Communication and Health Services Research at University Hospital Bonn (UKB). But a breast cancer diagnosis and successful treatment are often followed by long-term difficulties such as fatigue syndrome, which means a feeling of persistent tiredness, exhaustion and listlessness. Other after-effects of chemotherapy and the fear that the cancer will return can also limit productivity.

How do breast cancer patients succeed in returning to work? "There are numerous studies on this. Our long-term study now focuses on the post-return phase from the patient's perspective," says Hiltrop. Together with the head of the research center, Prof. Dr. Nicole Ernstmann, who is also a member of the Transdisciplinary Research Area "Life and Health" at the University of Bonn, and the German Cancer Society, the sociologist investigated how a total of 184 former breast cancer patients fared after returning to work over a period of five to six years after diagnosis.

From the perspective of breast cancer patients

The study focused on how satisfied the patients were with their occupational development since diagnosis. About half experienced at least one job change during the study period. "The main finding is that we found no relationship between the number of job changes and satisfaction, but that greater involuntariness of change was associated with lower satisfaction," Hiltrop says. "The results suggest that the quality of change matters more than the quantity." About 16 percent of job changes did not happen by choice. These changes included, for example, increased workload or retirement. The results suggest that the former breast cancer patients have difficulties in meeting the job requirements in the long term after their return, resulting in changes at work.

The research team's findings show what can contribute to satisfaction at work. "Providing a welcoming work environment and showing patients understanding and support can facilitate a satisfactory return to work," Hiltrop explains. This can help avoid involuntary job changes that are perceived as particularly drastic. The researchers conclude from the results that a satisfactory return to work and, in particular, remaining at work requires long-term support, for example because chemotherapies and aftercare have to be continued, there is a fear of a recurrence of the tumor, or fatigue symptoms have to be managed.

The current study is a follow-up study of the project "Strengthening patient competence: Breast cancer patients' information and training needs" (PIAT), which surveyed approximately 1000 breast cancer patients. The researchers interviewed the PIAT participants again 5-6 years after diagnosis. At four measurement points, surveys and interviews were used to explore the subjective assessment of health status, how often job changes occurred, and how fulfilling the job was. Socioeconomic data, such as age, number of children, and education, were also surveyed. Satisfaction with occupational development was associated with higher age, better perceived health status, and lower levels of involuntariness of job changes.

"Open communication with managers and colleagues about expectations and what can be accomplished is very important," explains Hiltrop. In addition, handling the situation more flexibly, can increase the likelihood that patients will be satisfied when they return to work.

Even though more and more vaccines against the coronavirus are being administered all over the world, many countries are still battling with outbreaks and face difficulties providing help to those in need.

One of those countries is Brazil. Here, they are facing a massive second wave outbreak, many daily deaths and instances of the health care systems collapsing. In the city of Manaus things have looked exceptionally bleak from December and through to the early spring.

The city was hit so hard by the first wave in 2020 that it was actually thought to be one of the few places in the world to have reached herd immunity. An estimated 75 percent of the population in the city had been infected. But then the second wave hit in November and December.

Now, a new study published in Science with collaborators from Brazil, the UK and University of Copenhagen has shed a light on why Manaus is facing these difficulties again.

'Our main explanation is that there is an aggressive variant of the coronavirus called P.1 which seems be the cause of their problems. Our epidemiological model indicates that P.1 is likely to be more transmissible than previous strains of coronavirus and likely to be able to evade immunity gained from infection with other strains', says corresponding author to the new study, Samir Bhatt, a researcher at the Department of Public Health at University of Copenhagen.

Emerged in November

The researchers used many forms of data from Manaus to characterize P.1 and its properties including 184 samples of genetic sequencing data. They find that genetically speaking P.1 is different from the previous strains of coronavirus. It has acquired 17 mutations including an important trio of mutations in the spike protein (K417T, E484K and N501Y).

'Our analysis shows that P.1 emerged in Manaus around November 2020. It went from not being detectable in our genetic samples to accounting for 87 percent of the positive samples in just seven weeks. It has since spread to several other states in Brazil as well as many other countries around the world', says Samir Bhatt.

Modelling using machine learning

The researchers then used an epidemiological model to estimate how transmissible P.1 seemed to be. As well as estimating signs of P.1 evading immunity gained from previous infection.

'Roughly speaking, our model incorporates many data sources such as mortality counts and genetic sequences and compares two different virus strains to see which one best explains the scenario that unfolded in Manaus. One was the 'normal coronavirus' and the other was dynamically adjusted using machine learning to best fit the actual events in Brazil', says Samir Bhatt.

This modeling allowed the researchers to conclude that P.1 is likely to be between 1.7 and 2.4 times more transmissible than non-P1-lineages of the coronavirus.

They also conclude that P.1 is likely to be able to evade between 10 and 46 percent of the immunity gained from infection with non-P.1 coronavirus.

'As researchers, we have to caution extrapolating these results to be applicable anywhere else in the world. However, our results do underline the fact that more surveillance of the infections and of the different strains of the virus is needed in many countries in order to get the pandemic fully under control', ends Samir Bhatt.

COLUMBUS, Ohio - Tiny molecules called nanobodies, which can be designed to mimic antibody structures and functions, may be the key to blocking a tick-borne bacterial infection that remains out of reach of almost all antibiotics, new research suggests.

The infection is called human monocytic ehrlichiosis, and is one of the most prevalent and potentially life-threatening tick-borne diseases in the United States. The disease initially causes flu-like symptoms common to many illnesses, and in rare cases can be fatal if left untreated.

Most antibiotics can't build up in high enough concentrations to kill the infection-causing bacteria, Ehrlichia chaffeensis, because the microbes live in and multiply inside human immune cells. Commonly known bacterial pathogens like Streptococcus and E. coli do their infectious damage outside of hosts' cells.

Ohio State University researchers created nanobodies intended to target a protein that makes E. chaffeensis bacteria particularly infectious. A series of experiments in cell cultures and mice showed that one specific nanobody they created in the lab could inhibit infection by blocking three ways the protein enables the bacteria to hijack immune cells.

"If multiple mechanisms are blocked, that's better than just stopping one function, and it gives us more confidence that these nanobodies will really work," said study lead author Yasuko Rikihisa, professor of veterinary biosciences at Ohio State.

The study provided support for the feasibility of nanobody-based ehrlichiosis treatment, but much more research is needed before a treatment would be available for humans. There is a certain urgency to coming up with an alternative to the antibiotic doxycycline, the only treatment available. The broad-spectrum antibiotic is unsafe for pregnant women and children, and it can cause severe side effects.

"With only a single antibiotic available as a treatment for this infection, if antibiotic resistance were to develop in these bacteria, there is no treatment left. It's very scary," Rikihisa said.

The research is published this week in Proceedings of the National Academy of Sciences.

The bacteria that cause ehrlichiosis are part of a family called obligatory intracellular bacteria. E. chaffeensis not only requires internal access to a cell to live, but also blocks host cells' ability to program their own death with a function called apoptosis - which would kill the bacteria.

"Infected cells normally would commit suicide by apoptosis to kill the bacteria inside. But these bacteria block apoptosis and keep the cell alive so they can multiply hundreds of times very rapidly and then kill the host cell," Rikihisa said.

A longtime specialist in the Rickettsiales family of bacteria to which E. chaffeensis belongs, Rikihisa developed the precise culture conditions that enabled growing these bacteria in the lab in the 1980s, which led to her dozens of discoveries explaining how they work. Among those findings was identification of proteins that help E. chaffeensis block immune cells' programmed cell death.

The researchers synthesized one of those proteins, called Etf-1, to make a vaccine-style agent that they used to immunize a llama with the help of Jeffrey Lakritz, professor of veterinary preventive medicine at Ohio State. Camels, llamas and alpacas are known to produce single-chain antibodies that include a large antigen binding site on the tip.

The team snipped apart segments of that binding site to create a library of nanobodies with potential to function as antibodies that recognize and attach to the Etf-1 protein and stop E. chaffeensis infection.

"They function similarly to our own antibodies, but they're tiny, tiny nano-antibodies," Rikihisa said. "Because they are small, they get into nooks and crannies and recognize antigens much more effectively.

"Big antibodies cannot fit inside a cell. And we don't need to rely on nanobodies to block extracellular bacteria because they are outside and accessible to ordinary antibodies binding to them."

After screening the candidates for their effectiveness, the researchers landed on a single nanobody that attached to Etf-1 in cell cultures and inhibited three of its functions. By making the nanobodies in the fluid inside E. coli cells, Rikihisa said her lab could produce them at an industrial scale if needed - packing millions of them into a small drop.

She collaborated with co-author Dehua Pei, professor of chemistry and biochemistry at Ohio State, to combine the tiny molecules with a cell-penetrating peptide that enabled the nanobodies to be safely delivered to mouse cells.

Mice with compromised immune systems were inoculated with a highly virulent strain of E. chaffeensis and given intracellular nanobody treatments one and two days after infection. Compared to mice that received control treatments, mice that received the most effective nanobody showed significantly lower levels of bacteria two weeks after infection.

With this study providing the proof of principle that nanobodies can inhibit E. chaffeensis infection by targeting a single protein, Rikihisa said there are multiple additional targets that could provide even more protection with nanobodies delivered alone or in combination. She also said the concept is broadly applicable to other intracellular diseases.

"Cancers and neurodegenerative diseases work in our cells, so if we want to block an abnormal process or abnormal molecule, this approach may work," she said.

RESEARCH TRIANGLE PARK, N.C. -- New Army-funded synthetic biology research manipulated micro-compartments in cells, potentially enabling bio-manufacturing advances for medicine, protective equipment and engineering applications.

Bad bacteria can survive in extremely hostile environments -- including inside the highly acidic human stomach--thanks to their ability to sequester toxins into tiny compartments.

In a new study, published in ACS Central Science, Northwestern University researchers controlled protein assembly and built these micro-compartments into different shapes and sizes, including long tubes and polyhedrons. Because this work illuminates how biological units, such as viruses and organelles, develop, it also could inform new ways to design medicine, synthetic cells and nano-reactors that are essential for nanotechnology.

"These results are an exciting step forward in our ability to design complex protein-based compartments," said Dr. Stephanie McElhinny, program manager at the U.S. Army Combat Capabilities Development Command, known as DEVCOM, Army Research Laboratory. "Being able to control the size and shape of these compartments could enable sophisticated bio-manufacturing schemes that are customized to support efficient production of complex molecules and multi-functional materials that could provide the future Army with enhanced uniforms, protective equipment and environmental sensors."

Further down the road, these insights potentially could lead to new antibiotics that target micro-compartments of pathogens while sparing good bacteria.

Researchers control protein assembly and build cell micro-compartments into different shapes and sizes that could lead to bio-inspired building blocks for various engineering applications.

"By carefully designing proteins to have specific mutations, we were able to control assembly of the proteins that form bacterial micro-compartments," said Dr. Monica Olvera de la Cruz, professor of materials science and engineering and chemistry at Northwestern who led the theoretical computation. "We used this also to predict other possible formations that have not yet been observed in nature."

Many cells use compartmentalization to ensure that various biochemical processes can occur simultaneously without interfering with one another. Made of proteins, these micro-compartments are a key to survival for a wide variety of bacterial species.

"Based on previous observations, we have known that the geometry of micro-compartments can be altered," said Dr. Danielle Tullman-Ercek, associate professor of chemical and biological engineering at Northwestern who led the experimental work. "But our work provides the first clues into how to alter them to achieve specific shapes and sizes."

To study these crucial compartments, the Northwestern team turned to Salmonella enterica, which rely on micro-compartments to break down the waste products of good bacteria in the gut. When the researchers genetically manipulated a protein isolated from Salmonella, they noticed the micro-compartments formed long tubes.

"We saw these weird, extended structures," Tullman-Ercek said. "It looked like they used the varying building blocks to form different shapes with different properties."

By coupling the mechanical properties of the compartment with the chemicals inside the compartment, Olvera de la Cruz and her team used theoretical computation to predict how different mutations led to different shapes and sizes. When six-sided proteins assembled together, they formed long tubes. When five-sided proteins assembled together, they formed soccer ball-shaped icosahedrons. The team also predicted that proteins could assemble into a triangular samosa shape, resembling the fried, South Asian snack.

Understanding this process could lead to bio-inspired building blocks for various engineering applications that require components of varying shapes and sizes.

"It's like building with Legos," Tullman-Ercek said. "It's not desirable to use the same shape block over and over again; we need different shapes. Learning from bacteria can help us build new and better structures at this microscopic scale."

Every day, the sun ejects large amounts of a hot particle soup known as plasma toward Earth where it can disrupt telecommunications satellites and damage electrical grids. Now, scientists at the U.S. Department of Energy's (DOE) Princeton Plasma Physics Laboratory (PPPL) and Princeton University's Department of Astrophysical Sciences have made a discovery that could lead to better predictions of this space weather and help safeguard sensitive infrastructure.

The discovery comes from a new computer model that predicts the behavior of the plasma in the region above the surface of the sun known as the solar corona. The model was originally inspired by a similar model that describes the behavior of the plasma that fuels fusion reactions in doughnut-shaped fusion facilities known as tokamaks.

Fusion, the power that drives the sun and stars, combines light elements in the form of plasma -- the hot, charged state of matter composed of free electrons and atomic nuclei -- that generates massive amounts of energy. Scientists are seeking to replicate fusion on Earth for a virtually inexhaustible supply of power to generate electricity.

The Princeton scientists made their findings while studying roped-together magnetic fields that loop into and out of the sun. Under certain conditions, the loops can cause hot particles to erupt from the sun's surface in enormous burps known as coronal mass ejections. Those particles can eventually hit the magnetic field surrounding Earth and cause auroras, as well as interfere with electrical and communications systems.

"We need to understand the causes of these eruptions to predict space weather," said Andrew Alt, a graduate student in the Princeton Program in Plasma Physics at PPPL and lead author of the paper reporting the results in the Astrophysical Journal.

The model relies on a new mathematical method that incorporates a novel insight that Alt and collaborators discovered into what causes the instability. The scientists found that a type of jiggling known as the "torus instability" could cause roped magnetic fields to untether from the sun's surface, triggering a flood of plasma.

The torus instability loosens some of the forces keeping the ropes tied down. Once those forces weaken, another force causes the ropes to expand and lift further off the solar surface. "Our model's ability to accurately predict the behavior of magnetic ropes indicates that our method could ultimately be used to improve space weather prediction," Alt said.

The scientists have also developed a way to more accurately translate laboratory results to conditions on the sun. Past models have relied on assumptions that made calculations easier but did not always simulate plasma precisely. The new technique relies only on raw data. "The assumptions built into previous models remove important physical effects that we want to consider," Alt said. "Without these assumptions, we can make more accurate predictions."

To conduct their research, the scientists created magnetic flux ropes inside PPPL's Magnetic Reconnection Experiment (MRX), a barrel-shaped machine designed to study the coming together and explosive breaking apart of the magnetic field lines in plasma. But flux ropes created in the lab behave differently than ropes on the sun, since, for example, the flux ropes in the lab have to be contained by a metal vessel.

The researchers made alterations to their mathematical tools to account for these differences, ensuring that results from MRX could be translated to the sun. "There are conditions on the sun that we cannot mimic in the laboratory," said PPPL physicist Hantao Ji, a Princeton University professor who advises Alt and contributed to the research. "So, we adjust our equations to account for the absence or presence of certain physical properties. We have to make sure our research compares apples to apples so our results will be accurate."

Discovery of the jiggling plasma behavior could also lead to more efficient generation of fusion-powered electricity. Magnetic reconnection and related plasma behavior occur in tokamaks as well as on the sun, so any insight into these processes could help scientists control them in the future.

Support for this research came from the DOE, the National Aeronautics and Space Administration, and the German Research Foundation. Research partners include Princeton University, Sandia National Laboratories, the University of Potsdam, the Harvard-Smithsonian Center for Astrophysics, and the Bulgarian Academy of Sciences.

PPPL, on Princeton University's Forrestal Campus in Plainsboro, N.J., is devoted to creating new knowledge about the physics of plasmas -- ultra-hot, charged gases -- and to developing practical solutions for the creation of fusion energy. The Laboratory is managed by the University for the U.S. Department of Energy's Office of Science, which is the single largest supporter of basic research in the physical sciences in the United States and is working to address some of the most pressing challenges of our time. For more information, visit https://energy.gov/science

Although plants may look fairly inactive to casual observers, research into plant biology has shown that plants can send each other signals concerning threats in their local environments. These signals take the form of airborne chemicals, called volatile organic compounds (VOCs), released from one plant and detected by another, and plant biologists have found that a diverse class of chemicals called terpenoids play a major role as airborne danger signals.

Past studies have shown that soybean and lima bean plants both release terpenoid signals that activate defense-related genes in neighboring plants of the same species, and this chemically induced gene activation can help the plants protect themselves from threats like herbivorous pests.

In recent years, scientists have realized that the capacity of these chemical signals to boost plant defense mechanisms could make them useful pest control tools for agriculture and horticulture. One such scientist is Prof. Gen-ichiro Arimura of the Tokyo University of Science, Japan. Prof. Arimura notes that "the development of agricultural technology to date has been largely reliant on the use of pesticides and chemical fertilizers, which has resulted in environmental pollution and the destruction of ecosystems." As a greener alternative to pesticides, terpenoid signaling molecules may help farmers continue their production of vital foodstuffs while lessening the associated environmental costs.

In pursuit of this goal, Prof. Arimura and his colleagues chose to investigate the terpenoid compound menthol, which is derived from mint leaves and can activate plant immune systems. The aim of this project, which the researchers describe in an article recently published in the journal Plant Molecular Biology, was to develop compounds that are structurally similar to menthol but improve upon menthol's ability to activate plant immune systems. The researchers therefore experimented with chemically modifying menthol by attaching amino acids, which are a structurally diverse set of compounds that living cells use to construct proteins. In total, the researchers synthesized six different menthol derivatives with attached amino acids.

The researchers then tested the resulting menthol derivatives to see whether the modified compounds could outperform unmodified menthol at activating plant defense mechanisms. To do this, they treated soybean leaves with either menthol or one of the six menthol derivatives to see which of the derivatives, if any, could outclass menthol itself at boosting the expression levels of two defense-related soybean genes after 24 hours of exposure. The found that only one of the modified compounds bested menthol, and this compound is called valine menthyl ester, or "ment-Val" for short.

The researchers found that spraying soybean leaves once with a ment-Val solution boosted expression of the defense-related genes for three days, and a second spraying on the fourth day worked to boost the expression of those genes again. These findings suggest that ment-Val could provide sustainable pest control for farmers growing soybeans. Further experiments showed that ment-Val also increased the expression of defense-related genes in other crops, including peas, tobacco, lettuce, and corn. Ment-Val also proved to be quite stable under various conditions, which suggests that farmers would probably not lose the compound to degradation during storage.

Overall, these results suggest that ment-Val could be extremely useful as an alternative to the chemical pesticides that so many farmers rely on. Prof. Arimura notes that spraying ment-Val may be an effective way "to reduce pest damage to soybeans and other crops." He has applied for a patent on ment-Val's use as a crop protection agent, and he predicts that the commercialization of ment-Val "will generate billions of yen in economic benefits through its usage by companies operating in the fields of horticulture and agriculture." He also notes that ment-Val's anti-inflammatory properties could make it useful for human medicine.

The future is certainly going to be exciting for research into menthol derivatives like ment-Val!

It's well established that infectious disease is the greatest threat to the endangered chimpanzees made famous by the field studies of Jane Goodall at Gombe National Park in Tanzania. Now, new research led by scientists at Emory University shows that nearly half of the fecal samples from wild chimpanzees contain bacteria that is resistant to a major class of antibiotics commonly used by people in the vicinity of the park.

The journal Pathogens published the findings.

"Our results suggest that antibiotic-resistant bacteria is actually spreading from people to non-human primates by making its way into the local watershed," says Thomas Gillespie, senior author of the study and associate professor in Emory's Department of Environmental Sciences and Rollins School of Public Health. "People are bathing and washing in the streams, contaminating the water with drug-resistant bacteria where wild chimpanzees and baboons drink."

The researchers tested for genes conferring resistance to sulfonamides -- drugs often used by people in the region to treat diarrheal diseases -- in fecal samples from humans, domestic animals, chimpanzees and baboons in and around Gombe National Park. They also tested stream water used by these groups.

Sulfonamide resistance appeared in 74 percent of the human samples overall, 48 percent of chimpanzee samples, 34 percent of baboon samples, and 17 percent of the domestic animal samples. Sulfonamide also showed up in 19 percent of the samples taken from streams shared by people, domestic animals and wildlife.

The researchers also tested all the groups in the study for genes conferring resistance to tetracycline -- another class of antibiotics that is used much less frequently by people in the vicinity, likely due to its greater expense and the fact that it is less available in the area. As expected, very few of the fecal samples from any of the groups, and none of the water samples from the streams, showed evidence of tetracycline resistance.

First author of the study is Michelle Parsons, who did the work as an Emory doctoral student in Environmental Sciences. Parsons has since graduated and works at the Centers for Disease Control and Prevention (CDC). Co-authors include researchers from the Jane Goodall Institute, the CDC, the University of Minnesota and Franklin and Marshall College.

Gillespie is a disease ecologist who helped pioneer the "One Health" approach to protect humans, ecosystems and biodiversity. His projects in Africa, including the collaboration with the Jane Goodall Institute in Tanzania, are focused on helping farmers subsisting amid fragmented forests co-exist with primates and other wildlife in ways that minimize the risk of pathogen exchange between species, known as "spillover." The virus that causes AIDS, for example, spilled over from chimpanzees to people.

"It's important to consider both sides of the story -- human health and well-being, as well as conservation of chimpanzees and other species," Gillespie says.

Human encroachment has taken a toll on the great apes, due to fragmented habitat and the exchange of pathogens. Today, the number of chimpanzees in Gombe National Park are down to about 95.

Diarrheal diseases are common in the area and people often turn to cheap sulfonamide antibiotics that are available without a prescription at small stores that act as informal pharmacies, selling drugs, soap and other necessities. Wild chimpanzees also suffer from wasting diseases that can be related to bacterial and other enteric pathogens that affect their ability to maintain calorie intake and absorb nutrients.

"The majority of people in our sampling harbored bacteria resistant to the sulfonamide medication they are taking," Gillespie says. "In those cases, they're spending their money on a drug that is not helping them get better. Overuse of such drugs creates the potential for more lethal, antibiotic-resistant 'super bugs' to emerge."

The research findings will now support the development of interventions. More guidance is needed locally regarding the proper use of antibiotics, Gillespie says. He adds that it is also important to improve hygiene for wash-related activities in area streams, as well as to improve disposal of human waste materials.

"By misusing antibiotics, people can actually harm not only themselves, but also the species they share an environment with," Gillespie says. "After drug-resistant bacteria jump into chimpanzees, it can further evolve with the chimpanzees and then spill back into humans. We need to be thinking about infectious diseases within evolutionary and ecological frameworks, something that's not often done in medicine."